Lot B5207 is one of 4 lots of Semaglutide we have release-tested for this catalog, scored on purity, quantity, and batch traceability. Independent third-party HPLC/MS verification of this lot is available on request.
9.8/10
A+ Excellent
Purity
3.8 / 4
Quantity
4.0 / 4
Identifier
2.0 / 2
The test score is calculated from purity (how much of the vial content is the expected compound, with values closest to 100% best and below 98% considered unacceptable), quantity (how close the measured amount is to the label claim, more than plus or minus 20% is unacceptable), and identifier (whether the batch this vial came from can be traced to a certificate).
Sample details
ProductSemaglutide 5mg
Sample typeSealed retail vial
Date received27 Oct 2025
Physical detailsPrinted label / aluminum crimp / amber cap
Test details
MethodRP-HPLC-UV + LC-MS
Tested on29 Oct 2025
Tested byRTT Peptides QC
ReportQC-23029
Score breakdown
Purity
99.59%
3.8 / 4
Measured purity by RP-HPLC-UV against the ≥ 98.0% release specification. Net peptide content 91.8%.
Quantity
5.1mg
4.0 / 4
Label claim 5mg, measured 5.1mg, divergence +2.0%. Within plus or minus 5% of label scores full marks.
Identifier
B5207
2.0 / 2
Batch identifier printed on the vial and traceable to this certificate, so the lot can be verified end to end.
Analytical release certificate · RP-HPLC-UV with LC-MS confirmation Independent third-party verification available on request.
ReportQC-23029Issued2025-10-30Page1 of 1
Certificate of AnalysisSemaglutide · Lot B5207
SampleSemaglutide
Issued forRTT Peptides
Lot / BatchB5207
Label claim5mg
CAS910463-68-2
AppearanceWhite lyophilized powder
Date received2025-10-27
Date analyzed2025-10-29
01 Purity by RP-HPLC-UV
Figure 1 · Reverse-phase HPLC-UV chromatogram at 214 nm. C18, 2.7 µm; gradient 0.1% TFA in water / acetonitrile at 1.0 mL/min.
Purity by RP-HPLC-UV: component retention time, peak area, and area percent
#
Component
RT (min)
Area (mAU·s)
Area %
1
RRT 0.47
4.06
10.9
0.26
2
RRT 0.54
4.68
2.5
0.06
3
RRT 0.65
5.64
3.8
0.09
4
Semaglutide ▸ main
8.67
4182.8
99.59
✓
Purity (HPLC) 99.59% · net peptide content 91.8% · meets the ≥ 98.0% release specification.
02 Identity by LC-MS
Expected average mass4113.58 Da
Observed mass4113.84 Da
✓
Identity confirmed · observed mass conforms to the expected structure of Semaglutide.
03 Summary of results
Summary of results: test, method, specification, result, and outcome
Test
Method
Specification
Result
Outcome
Purity
RP-HPLC-UV, 214 nm
≥ 98.0 %
99.59 %
✓ Pass
Identity
LC-MS (ESI+)
Consistent with structure
Confirmed
✓ Pass
Net peptide content
By difference
Report
91.8 %
n/a
Appearance
Visual
White lyophilized powder
Conforms
✓ Pass
Reconstitution
Bacteriostatic water
Clear, particulate-free
Conforms
✓ Pass
The submitted lot conforms to identity and meets the stated purity specification. Analysis was performed by RP-HPLC-UV with LC-MS confirmation. Results relate only to the lot as received and tested and do not constitute a certificate of any third party.
Quality Assurance · RTT Peptides
Issued under the RTT Peptides quality program; this is the vendor's own release certificate. Independent third-party HPLC/MS verification of any lot is available on request. Products are supplied strictly as research reagents for in-vitro laboratory use only, not for human or animal consumption. Report QC-23029 · RP.
This is the vendor's own release certificate. Independent third-party HPLC/MS verification of this lot is available on request.
Research use only
RTT Peptides sells laboratory research reagents. They are not for human or animal consumption. Please confirm the statements below to place your order.
